row of colorful sticky vector notes with pins / reihe bunter vektor klebezettel mit stecknadel kaufen sie diese vektorgrafik und finden sie hnliche vektorgrafiken auf adobe stock colored sticky papers with vector transparency kaufen sie diese vektorgrafik und finden sie hnliche vektorgrafiken auf adobe stock Sticky end ligation with larger insert (.kb vector + .kb insert) . blunt end ligation ligation efficiency was severely decreased (x) by . using dna fragments that have been exposed to uv during the gel extraction procedure (can avoid by blind excision, or by using blacklight or nm uv transilluminator instead of the usual nm type) . using the neb quick ligation kit . the cloning strategy. higher yields of the right recombinant are obtained when the vector and insert have been prepared using two restriction enzymes and the digested vector has been gelpurified before the ligation reaction (as shown in the figure). the ligation of bluntended fragments is less effective than that of stickyended ones.
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